General Cell Based Assays
Eurofins Discoverx PathHunter Human TSHR(L) β-Arrestin-1 Cell-Based Assay Kit (U2OS)
The PathHunter Human TSHR(L) β-Arrestin-1 Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure TSHR(L) (GPCR) activity via recruitment of β-Arrestin-1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Bioassay Systems EnzyChrom™ Starch Assay Kit. For quantitative determination of starch and evaluation of drug effects on starch metabolism.
For quantitative determination of starch and evaluation of drug effects on starch metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL starch for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological, agriculture, food etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 µg/mL.
Bioassay Systems EnzyChrom™ Sialic Acid Assay Kit. For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 µM for fluorimetric assays. Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: OD, FL: 20, 2 µM.
Bioassay Systems EnzyChrom™ Acetate Assay Kit. For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.
For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism. Key Features: Sensitive. Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays. Simple. The assay involves adding a single working reagent, incubation for 30 min at room temperature and read. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, food, agriculture and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.13 mM.
Bioassay Systems QuantiChrom™ Acetylcholinesterase Assay Kit. For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors.
For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors. Key Features: Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD412nm. Samples: Blood, serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 10 U/L.
Bioassay Systems EnzyChrom™ Creatine Assay Kit. For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.
For quantitative determination of creatine and evaluation of drug effects on creatine metabolism. Key Features: High sensitivity and wide linear range. Use 10 µL sample. Linear detection range 4 to 1000 µM (colorimetric) or 0.5 to 50 µM (fluorimetric). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes. Method: OD570nm, or FL530/590nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 4 µM.
Bioassay Systems QuantiChrom™ Creatinine Assay Kit. For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism.
For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).
Cell Biolabs Inc CytoSelect™ 96-Well Anoikis Assay Kit (Colorimetri/Fluorometric)
Adhesion to the extracellular matrix is essential for the survival of many adherent cells. Apoptosis that results from the loss of cell adhesion to the ECM, or inappropriate adhesion, is defined as “anoikis”. A common feature of carcinoma development is the ability of transformed cells to survive under anchorage independent or “spheroid” growth conditions. Resistance to anoikis has been shown to be involved in the loss of cell homeostasis, cancer growth, and metastasis. Preventing anoikis is a major goal in the development of cell transplantation techniques, including the therapeutic use of progenitor cells. The CytoSelect™ 96-well Anoikis Assay Kit provides a colorimetric and fluorometric format to measure anchorage-independent growth and monitoring anoikis propelled cell death. The kit contains sufficient reagents for 96 samples in a hydrogel-coated 96-well plate. Live cells are detected with MTT or Calcein AM. Cell death is detected with the Ethidium Homodimer (EthD-1).
Cell Biolabs Inc Total Bile Acid Assay Kit (Fluorometric), 100 assays
Determining circulatory levels of bile acids can be used to identify or diagnose certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. Cell Biolabs’ Total Bile Acid Assay Kit is a simple fluorometric assay that measures the amount of total bile acid present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay is based on an enzyme driven reaction: when bile acids are incubated in the presence of 3α-hydroxysteroid dehydrogenase (3α-HSD) and NAD+, NAD+ is converted to its reduced form NADH. Diaphorase then uses NADH to reduce resazurin to resorufin which is then detected fluorometrically. Sample bile acid concentrations are determined by comparison with a known bile acid standard. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples.
Cell Biolabs Inc ViraDuctin™ AAV Transduction Kit, 50 transductions
AAV can infect both dividing and non-dividing cells and can be maintained in the human host cell, creating the potential for genome integration. Because AAV is a naturally defective virus, requiring provision of several factors in trans for productive infection, it is considered the safest viral vector to use. The AAV transduction process includes viral binding and entry, intracellular trafficking, nuclear transport, and viral second strand DNA synthesis. The viral second strand DNA synthesis has been shown to be the rate limiting step, which leads to inefficient transduction by AAV vectors. ViraDuctin™ AAV Transduction Kit is a proprietary formulation designed to increase the transduction efficiency of AAV vectors in a variety of cell types. This kit provides the following advantages: (1) Higher transduction efficiency in many cell types, (2) Easy to use, and (3) It is ideal for transduction of nonpermissive cells such as primary cells and stem cells. 50 transductions/kit.
Eurofins Discoverx PathHunter Human IL-10 (IL10RA/IL10RB) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-10 (IL10RA/IL10RB) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL10RA/IL10RB. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.